Certificates
SLEEP is manufactured in the European Union in a facility that holds the following certifications.
- GMP (Good Manufacturing Practice) – manufacturing facility, food supplement manufacture.
- ISO 9001 – manufacturing facility, quality management.
- ISO 22000 – manufacturing facility, food safety management.
- Non-GMO – SLEEP, ingredients and finished product.
- Vegan – SLEEP contains no animal-derived ingredients, confirmed by the manufacturer.
Certificates are held by the manufacturer and the product; copies will be published here with the manufacturer's permission.
Batch testing
Every batch of SLEEP is tested by an independent third-party laboratory before release. The report for the current batch will be published here.
- What is tested: identity and amount of key actives, heavy metals, microbiology.
Request the certificates
PDFs will be published here as soon as they are uploaded.
A certificate of analysis (COA) is the laboratory's record of what a specific batch was tested for and what was found. It does not describe effects; it confirms that what is on the label is in the sachet and that the batch meets safety limits.
The research behind the amounts
WITH ME chooses the amount of each ingredient from published, peer-reviewed studies, and matches the study amount where the formula allows. The studies below tested specific ingredients, doses and groups of participants, not SLEEP. Research on individual ingredients does not establish how the complete SLEEP formula will work for you.
L-Glycine – 3,000 mg per sachet
A placebo-controlled study in healthy volunteers whose sleep was restricted tested 3 g of glycine before bedtime over three nights. It reported improvements in next-day fatigue and psychomotor vigilance. SLEEP contains 3 g of glycine per sachet, but this study does not establish the effects of the complete formula. Read the glycine study.
Magnesium – 200 mg per sachet (from magnesium bisglycinate)
A randomized, placebo-controlled trial in 155 adults reporting poor sleep tested magnesium bisglycinate providing 250 mg of elemental magnesium daily for four weeks. It found a small improvement in self-reported insomnia severity compared with placebo. SLEEP provides 200 mg of elemental magnesium per sachet, so the study dose differs. This finding does not establish the same benefit for SLEEP. Read the magnesium study.
Studies on the ingredients in SLEEP
The table lists published studies on individual ingredients that are in SLEEP: who was studied, the dose and form used, and what the researchers measured. The last column shows the amount of that ingredient in one SLEEP sachet, for comparison. Where the study form or dose differs from SLEEP, both are shown.
| Ingredient | Study | Dose and form studied | What was measured | In one SLEEP sachet |
|---|---|---|---|---|
| Ashwagandha root extract | Salve et al., Cureus 2019; 58 healthy adults reporting stress, 8 weeks | 250 mg or 600 mg a day | Serum cortisol and self-rated sleep quality, compared with placebo | 250 mg KSM-66® root extract |
| Glycine | Yamadera et al., Sleep Biol Rhythms 2007 | 3 g before bed | Subjective sleep quality and sleep recordings (polysomnography) | 3,000 mg |
| Glycine | Bannai and Kawai, J Pharmacol Sci 2012 (review) | Review of earlier studies | Peripheral blood flow and core body temperature | 3,000 mg |
| L-Ornithine | Miyake et al., Nutr J 2014; healthy workers, 8 weeks | 400 mg a day | Serum cortisol, cortisol/DHEA-S ratio and perceived sleep quality, compared with placebo | 750 mg |
| Montmorency tart cherry | Howatson et al., Eur J Nutr 2012; 20 adults, 7 days | Tart cherry juice concentrate | Urinary melatonin, total sleep time and sleep efficiency | 800 mg CherryPURE® EQ powder (different form) |
| Montmorency tart cherry | Levers et al., J Int Soc Sports Nutr 2016; trained runners | 480 mg powder a day | Muscle soreness and markers of muscle damage after a half-marathon | 800 mg CherryPURE® EQ powder |
| L-Theanine | Hidese et al., Nutrients 2019; 30 adults, 4 weeks | 200 mg a day | Stress-related symptoms and sleep-quality scores | 100 mg (from green tea extract) |
| Chamomile | Chang and Chen, J Adv Nurs 2016; 80 postnatal women, 2 weeks | Chamomile tea | Sleep-quality and mood scores, compared with a control group | 800 mg flower extract (different form) |
| Bacillus coagulans MTCC 5856 | Majeed et al., Nutr J 2016; adults with IBS, 90 days | 2 billion spores a day | Digestive symptom scores, compared with placebo | 2 billion CFU (LactoSpore®) |
| Bacillus coagulans MTCC 5856 | Shinde et al., J Funct Foods 2019; laboratory study | Spores in simulated digestion | Share of spores surviving simulated digestion | 2 billion CFU |
| Reishi (Ganoderma lucidum) | Tang et al., J Med Food 2005; 132 adults with neurasthenia, 8 weeks | 5.4 g polysaccharide extract a day | Fatigue and wellbeing scores, compared with placebo | 1,000 mg fruiting-body extract (300 mg polysaccharides) |
| Reishi | Cui et al., J Ethnopharmacol 2012; animal study (rats) | Reishi extract | Sleep time in rats | 1,000 mg |
| Reishi | Yao et al., Sci Rep 2021; animal study (mice) | Reishi extract | Sleep, serotonin and gut bacteria in mice | 1,000 mg |
Other published studies on these ingredients
These studies looked at other outcomes and populations. They are listed for completeness and say nothing about SLEEP.
| Ingredient | Study | Dose and form studied | What was measured | In one SLEEP sachet |
|---|---|---|---|---|
| Bacillus coagulans MTCC 5856 | Majeed et al., Food Nutr Res 2018; 40 adults with IBS and depression, 90 days | 2 billion spores a day | Depression scores, IBS quality of life and daytime sleepiness (Epworth scale), compared with placebo | 2 billion CFU |
| Reishi (Ganoderma lucidum spore extract) | Qin et al., Front Pharmacol 2024; animal study (rat model of sporadic Alzheimer's disease) | Reishi spore extract | Sleep architecture and GABA activity in a rat model; animal study | 1,000 mg fruiting-body extract (different form) |
These studies looked at individual ingredients at the doses and forms listed, not at SLEEP. They describe research, not the effect of any product. Food supplements are not intended to diagnose, treat or prevent any disease.
How strong is this evidence?
Most of the reishi sleep data come from animal studies; there is not yet a human trial with sleep as its main outcome. The human trials on Bacillus coagulans MTCC 5856 are small, were funded by the ingredient's manufacturer, and did not have sleep as their main outcome. Several studies used a different dose or form from SLEEP; both are shown in the table.
The full sleep guide, with all sources: Sleeping Beauty
Sources
- Bannai M, et al. The effects of glycine on subjective daytime performance in partially sleep-restricted healthy volunteers. Front Neurol. 2012;3:61. doi:10.3389/fneur.2012.00061
- Randomised, placebo-controlled trial of magnesium bisglycinate (250 mg elemental magnesium a day, 4 weeks) in 155 adults reporting poor sleep. Nat Sci Sleep. doi:10.2147/nss.s524348
- Bannai M, Kawai N. New therapeutic strategy for amino acid medicine: glycine improves the quality of sleep. J Pharmacol Sci. 2012;118(2):145–148.
- Chang SM, Chen CH. Effects of an intervention with drinking chamomile tea on sleep quality and depression in sleep disturbed postnatal women. J Adv Nurs. 2016;72(2):306–315.
- Cui XY, et al. Extract of Ganoderma lucidum prolongs sleep time in rats. J Ethnopharmacol. 2012;139(3):796–800.
- Hidese S, et al. Effects of L-theanine administration on stress-related symptoms and cognitive functions in healthy adults. Nutrients. 2019;11(10):2362.
- Howatson G, et al. Effect of tart cherry juice (Prunus cerasus) on melatonin levels and enhanced sleep quality. Eur J Nutr. 2012;51(8):909–916.
- Levers K, et al. Effects of powdered Montmorency tart cherry supplementation on acute endurance exercise performance in aerobically trained individuals. J Int Soc Sports Nutr. 2016;13:22.
- Majeed M, et al. Bacillus coagulans MTCC 5856 supplementation in the management of diarrhea predominant irritable bowel syndrome. Nutr J. 2016;15:21.
- Majeed M, et al. Bacillus coagulans MTCC 5856 for the management of major depression with irritable bowel syndrome: a randomised, double-blind, placebo controlled, multi-centre, pilot clinical study. Food Nutr Res. 2018;62:1218.
- Miyake M, et al. Randomised controlled trial of the effects of L-ornithine on stress markers and sleep quality in healthy workers. Nutr J. 2014;13:53.
- Qin Y, et al. Ganoderma lucidum spore extract improves sleep disturbances in a rat model of sporadic Alzheimer's disease. Front Pharmacol. 2024;15:1390294.
- Salve J, et al. Adaptogenic and anxiolytic effects of ashwagandha root extract in healthy adults. Cureus. 2019;11(12):e6466.
- Shinde T, et al. Probiotic Bacillus coagulans MTCC 5856 spores exhibit excellent in-vitro functional efficacy in simulated gastric survival, mucosal adhesion and immunomodulation. J Funct Foods. 2019;52:100–108.
- Tang W, et al. A randomized, double-blind and placebo-controlled study of a Ganoderma lucidum polysaccharide extract in neurasthenia. J Med Food. 2005;8(1):53–58.
- Yamadera W, et al. Glycine ingestion improves subjective sleep quality in human volunteers, correlating with polysomnographic changes. Sleep Biol Rhythms. 2007;5(2):126–131.
- Yao C, et al. Ganoderma lucidum promotes sleep through a gut microbiota-dependent and serotonin-involved pathway in mice. Sci Rep. 2021;11(1).
How to read this research
When might I notice a difference, and how long should I try it? The research cited here does not establish an onset time or a recommended trial period for SLEEP. Study duration is not the same as time to first effect. Follow the label directions rather than increasing your serving to match a research dose.
Will everyone notice the same results? Study averages are not a prediction of your experience. A noticeable benefit is not guaranteed.
Falling asleep or staying asleep? These are different outcomes. The ingredient studies described above do not establish that SLEEP helps you fall asleep faster, prevents night-time waking or keeps you asleep longer.
Do I need to cycle it? The studies cited here do not establish a cycling schedule for SLEEP or demonstrate that long-term, uninterrupted use of the complete formula is suitable.
What if I work night shifts? The studies cited here do not establish SLEEP's effectiveness or a dosing schedule for night-shift workers. Ask your doctor or pharmacist for advice about use with your sleep schedule.
OUR APPROACH
How we build our formula.
NAMED INGREDIENTS
Each ingredient is listed by form and source, no proprietary blends.
DISCLOSED AMOUNTS
Every amount per serving is on the label.
MELATONIN-FREE
No melatonin and no stimulants.
REVIEWED OVER TIME
We review the formula as new information becomes available.
The full list of ingredients, forms and amounts is on the Ingredients page.
WHY SLEEP MATTERS
Why a regular sleep routine matters.
Adults are generally advised to sleep 7 or more hours a night on a regular schedule. Good sleep habits include:
- Going to bed and getting up at about the same time every day.
- Dimming lights and putting screens away in the hour before bed.
- Finishing large meals, caffeine and alcohol well before bedtime.
- Keeping the bedroom dark, quiet and cool.
- Getting daylight and movement during the day.
These habits matter more than any supplement.
Testing and research, answered.
What the certificates, the batch testing and the studies do and do not tell you.
Is SLEEP third-party tested?
Yes. Every batch of SLEEP is tested by an independent laboratory before release. The report for the current batch will be published on this page.
What certifications does the facility that makes SLEEP have?
GMP, ISO 9001 and ISO 22000. SLEEP itself is certified non-GMO and is manufactured in the European Union.
What is a certificate of analysis?
A laboratory's record of what a specific batch was tested for and what was found. It confirms that the label amounts are present and that the batch meets safety limits; it does not describe effects.
Which studies is SLEEP based on?
The amount of each ingredient is chosen from published, peer-reviewed studies. The studies for glycine and magnesium are summarised and linked on this page; they tested single ingredients, not SLEEP.
Does research on an ingredient prove that SLEEP works?
No. A study tests a specific ingredient, dose and group of participants. It does not establish how the complete SLEEP formula will work for you.
Is SLEEP a medicine?
No. SLEEP is a food supplement. It is not intended to treat, prevent or cure any condition, and it is not a substitute for a varied, balanced diet, a healthy lifestyle or medical advice.
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